Fagus Sylvatica Research Articles

Coordinated hydraulic traits influence the two phases of time to hydraulic failure in five temperate tree species differing in stomatal stringency.

Worldwide, forests are increasingly exposed to extreme droughts causing tree mortality. Because of the complex nature of the mechanisms involved, various traits have been linked to tree drought responses with contrasting results. This may be due to species-specific strategies in regulating water potential, a process that unfolds in two distinct phases: a first phase until stomatal closure, and a second phase until reaching lethal xylem hydraulic thresholds. We conducted dry-down experiments with five broadleaved temperate tree species differing in their degree of isohydry to estimate the time to stomatal closure (tsc) and subsequent time to critical hydraulic failure (tcrit). We measured various traits linked to tree drought responses, such as the water potentials at turgor loss point (Ptlp), stomatal closure (Pgs90) and 12, 50 and 88% loss of xylem hydraulic conductance (P12, P50, P88), hydraulic capacitance (C), minimum leaf conductance (gmin), hydroscape area (HSA) and hydraulic safety margins (HSM). We found that Pgs90 followed previously recorded patterns of isohydry and was associated with HSA. Species ranked from more to less isohydric in the sequence Acer pseudoplatanus<Betula pendula<Tilia cordata<Sorbus aucuparia<Fagus sylvatica. Their degree of isohydry was associated with leaf safety (Ptlp and gmin), drought avoidance (C) and tsc, but decoupled from xylem safety (HSM and P88) and tcrit. Regardless of their stomatal stringency, species with wider HSM and lower P88 reached critical hydraulic failure later. We conclude that the duration of the first phase is determined by stomatal regulation, while the duration of the second phase is associated with xylem safety. Isohydry is thus linked to water use rather than to drought survival strategies, confirming the proposed use of HSA as a complement to HSM for describing plant drought responses before and after stomatal closure.

Determining occupational exposure to inhalable wood dust in motor manual felling and processing

ABSTRACT The occupational exposure to softwood and hardwood wood dust was determined during forestry work in timber stands. In the determination of inhalable wood dust, three tree species (Fagus sylvatica, Quercus sp. and Pinus brutia) and three chainsaw operating parameters were taken into account (in total six assessment indicators). The first operating parameter was the chainsaw operating under normal conditions (regular chain sharpening). The second and third operating parameters were improper chainsaw maintenance (irregular chain sharpening) and low throttling pressing (operating the chainsaw at low engine speeds), respectively. The determination of inhalable wood dust concentrations from the ambient air was performed using the Inhalable Organic Matter (IOM) sampler, manufactured by SKC Button Sampler (Dorset, United Kingdom). Out of a total of 108 concentration cases of inhalable wood dust, 44 (40.74%) showed concentrations greater than 3.00 mg·m−3, while in 10 (9.26%) cases, the dust concentration exceeded 5 mg·m−3. The eight-hour time-weighted average exposure to wood dust ranged from 0.99 mg·m−3 to 12.52 mg·m−3, with an average of 4.22 mg·m−3. The results indicated that the increase in wood dust concentration was dependent on improper chainsaw maintenance, while low throttling pressing reduces dust concentration levels. Additionally, an increase in wood dust concentration was observed in species with thick bark (Pinus brutia and Quercus petraea).

Detecting Pathogenic Phytophthora Species Using Volatile Organic Compounds

There are several highly damaging Phytophthora species pathogenic to forest trees, many of which have been spread beyond their native range by the international trade of live plants and infested materials. Such introductions can be reduced through the development of better tools capable of the early, rapid, and high-throughput detection of contaminated plants. This study utilized a volatilomics approach (solid-phase microextraction coupled to gas chromatography–mass spectrometry) to differentiate between several Phytophthora species in culture and discriminate between healthy and Phytophthora-inoculated European beech and pedunculate oak trees. We tentatively identified 14 compounds that could differentiate eight Phytophthora species from each other in vitro. All of the Phytophthora species examined, except Phytophthora cambivora, uniquely produced at least one compound not observed in the other species; however, most detected compounds were shared between multiple species. Phytophthora polonica had the most unique compounds and was the least similar of all the species examined. The inoculated seedlings had qualitatively different volatile profiles and could be distinguished from the healthy controls by the presence of isokaurene, anisole, and a mix of three unknown compounds. This study supports the notion that volatiles are suitable for screening plant material, detecting tree pathogens, and differentiating between healthy and diseased material.

Improving the Decay Resistance of Wood through the Fixation of Different Nanoparticles Using Silica Aerogel

Nowadays, the protection of wood is becoming more important with the increasing demand for durable wood, in addition to its limited accessibility. One possible way to increase the durability is the use of nanoparticles, which can be effective even with a low intake of active ingredients. However, avoiding their leaching is a challenge. A possible solution to leaching is the use of silica aerogel as a fixative. This study investigated the use of mesoporous silica aerogel against the leaching of different nanoparticles under laboratory conditions. Tests were performed involving beech (Fagus sylvatica) and Scots pine (Pinus sylvestris) sapwood, using Trametes versicolor as a white rot and Coniophora puteana as a brown rot fungus. The results show that the subsequent treatment of the wood with mesoporous silica aerogel effectively fixed the nanoparticles in wood. The durability of the samples without aerogel significantly decreased as a result of leaching, whereas the resistance of the samples treated with aerogel decreased only slightly. However, the silica aerogel modification itself caused the leaching of silver nanoparticles, which is a limitation in the use of this method for the fixation of nanoparticles.

Forest top canopy bacterial communities are influenced by elevation and host tree traits

BackgroundThe phyllosphere microbiome is crucial for plant health and ecosystem functioning. While host species play a determining role in shaping the phyllosphere microbiome, host trees of the same species that are subjected to different environmental conditions can still exhibit large degrees of variation in their microbiome diversity and composition. Whether these intra-specific variations in phyllosphere microbiome diversity and composition can be observed over the broader expanse of forest landscapes remains unclear. In this study, we aim to assess the variation in the top canopy phyllosphere bacterial communities between and within host tree species in the temperate European forests, focusing on Fagus sylvatica (European beech) and Picea abies (Norway spruce).ResultsWe profiled the bacterial diversity, composition, driving factors, and discriminant taxa in the top canopy phyllosphere of 211 trees in two temperate forests, Veluwe National Parks, the Netherlands and Bavarian Forest National Park, Germany. We found the bacterial communities were primarily shaped by host species, and large variation existed within beech and spruce. While we showed that there was a core microbiome in all tree species examined, community composition varied with elevation, tree diameter at breast height, and leaf-specific traits (e.g., chlorophyll and P content). These driving factors of bacterial community composition also correlated with the relative abundance of specific bacterial families.ConclusionsWhile our results underscored the importance of host species, we demonstrated a substantial range of variation in phyllosphere bacterial diversity and composition within a host species. Drivers of these variations have implications at both the individual host tree level, where the bacterial communities differed based on tree traits, and at the broader forest landscape level, where drivers like certain highly plastic leaf traits can potentially link forest canopy bacterial community variations to forest ecosystem processes. We eventually showed close associations between forest canopy phyllosphere bacterial communities and host trees exist, and the consistent patterns emerging from these associations are critical for host plant functioning.

Význam buku lesního ve střední Evropě v období klimatické změny: přehled současných poznatků

In the period of ongoing global climate change, ecosystems, including Central European forests, face significant challenges such as summer droughts and heat waves, which increase the climate risks also for the European beech (Fagus sylvatica L.), an important and dominant broadleaved tree species in this region. This article provides a comprehensive review of current knowledge on the adaptation strategies of European beech in response to changing climatic conditions, highlighting its ecological demands, development of taxonomy, ecological and economic importance and exploring its resilience, growth characteristics, and challenges such as water regime, pests, diseases, and the impact of climate change. A key finding is that despite the various stressors affecting European beech, adaptive management and the use of mixed forest stands can significantly contribute to its conservation and sustainable use. The importance of integrating the genetic diversity and adaptive capacity of European beech into forestry practices is emphasised. The review of 203 studies highlights the need for further research focused on understanding the interactions between European beech and a changing climate in order to develop more effective strategies for its conservation and sustainable management in the future.

Phenological response of European beech (Fagus sylvatica L.) to climate change in the Western Carpathian climatic-geographical zones

IntroductionThe paper analyzes the results of 26 years (1996–2021) of phenological observations of the vegetative organs of European beech (Fagus sylvatica L.) in the Western Carpathians. It evaluates the influence of the heterogeneity of this territory, including relief and elevation, based on climatic-geographical types.MethodsPhenological stages, including leaf unfolding, full leaves, leaf coloring, and leaf fall, were monitored at 40 phenological stations across eight elevation zones. The study assesses trends in the occurrence of phenological stages, the length of the growing season, and phenological elevation gradients.ResultsThe results indicate a statistically significant earlier onset of spring phenological phases and delay in autumn phases, resulting in an average extension of the beech growing season by 12 days. Our findings confirm that the lengthening of the growing season due to warming, as an expression of climate change, is predominantly attributed to the warming in the spring months. The detected delayed onset of autumn phenophases was not due to warming in the autumn months, but other environmental factors influence it. The trend of elongation of the growing season (p&amp;lt;0.01) is observed in all elevation zones, with a less significant trend observed only in zones around 400 and 600 m a.s.l, signaling changes in environmental conditions across most of the elevation spectrum. Moreover, the heterogeneity of climatic-geographical types within each elevation zone increases the variability in the duration of the growing season for sites with similar elevations. By extending the growing season, it is assumed that the beech area will be changed to locations with optimal environmental conditions, especially in terms of adverse climatic events (late spring frosts, drought) during the growing season. The phenological elevation gradients reveal an earlier onset of 2.2 days per 100 m for spring phenophases and a delay of 1.1–2.9 days per 100 m for autumn phenophases.DiscussionThese findings highlight the specific environmental conditions of European beech in the Western Carpathians and their potential for anticipating changes in its original area. Additionally, these observations can aid in forecasting the further development of phenological manifestations related to climate change.

Molded Plywood with Proportions of Beech Bark in Adhesive Mixtures: Production on an Industrial Scale.

Molded plywood is used for furniture components such as seats, backrests, or integral seat shells, and it must be durable and harmless to health. Molded plywood is made with urea-formaldehyde (UF) adhesives; therefore, the issue of the fillers used in them is important. The potential of using ground beech (Fagus sylvatica L.) bark as an eco-friendly additive in UF adhesives for molded plywood manufacturing was investigated in this work. Wheat flour was used as a reference filler. The beech bark (BB) level as a filler was 10%, a value verified under laboratory conditions. Nine-layer flat and molded plywood were produced under industrial conditions from beech veneers bonded with a UF adhesive mixture. The mechanical (bending strength and bonding quality) and physical (swelling and absorbency values after 2 and 24 h) properties of the industrially fabricated molded plywood were evaluated and compared with the European standard requirements (EN 310 and EN 314-2). The mechanical properties of the molded plywood with the addition of BB in the adhesive mixture were acceptable and met these standards' requirements. The positive effect of BB in the UF adhesive mixture on a reduction in formaldehyde emissions from the molded plywood was also confirmed. BB, considered to be wood-processing industry waste or a by-product, has significant potential to be used as a filler in UF resins for molded plywood production, providing an environmentally friendly, inexpensive solution for the industrial valorization of bark as a bio-based formaldehyde scavenger.

Noise Analysis for Unbiased Tree Diameter Estimation from Personal Laser Scanning Data

Personal laser scanning devices employing Simultaneous Localization and Mapping (SLAM) technology have rightfully gained traction in various applications, including forest mensuration and inventories. This study focuses the inherent stochastic noise in SLAM data. An analysis of noise distribution is performed in GeoSLAM ZEB Horizon for point clouds of trees of two species, Norway spruce and European beech, to mitigate bias in diameter estimates. The method involved evaluating residuals of individual 3D points concerning the real tree surface model based on TLS data. The results show that the noise is not symmetrical regarding the real surface, showing significant negative difference, and moreover, the difference from zero mean significantly differs between species, with an average of −0.40 cm for spruce and −0.44 cm for beech. Furthermore, the residuals show significant dependence on the return distance between the scanner and the target and the incidence angle. An experimental comparison of RANSAC circle fitting outcomes under various configurations showed unbiased diameter estimates with extending the inlier tolerance to 5 cm with 2.5 cm asymmetry. By showing the nonvalidity of the assumption of zero mean in diameter estimation methods, the results contribute to fill a gap in the methodology of data processing with the widely utilized instrument.

Rapid assessment of beech leaf disease in Fagus sylvatica buds

AbstractThe European beech (Fagus sylvatica) is threatened by the foliar nematode Litylenchus crenatae subsp. mccannii (Lcm), the causal agent of beech leaf disease (BLD). Thus far, the majority of studies regarding BLD have focused on American beech (F. grandifolia). To better determine the impact of Lcm in buds of European beech, a total of 54 buds were collected from naturally symptomatic trees. Here, we characterized for the first time the bud scale morphology of two different cultivars of F. sylvatica infected with Lcm. Detailed observations of asymptomatic and symptomatic bud scales provided insight into the physical changes and arrangements of cells in the bud scale, shedding light on the dynamic processes occurring during Lcm infection. In addition, we evaluated the suitability of using the bud scale morphology for the early detection of BLD and Lcm in naturally infected buds. The distinct cellular arrangement of symptomatic bud scales cells (i.e., asymmetric pattern of enlarged cells) provides a rapid and visual, user‐friendly methodology to prematurely diagnose BLD symptoms within the buds, as well as the detection of associated nematodes.

Chromosome-scale genomes of Quercus sichourensis and Quercus rex provide insights into the evolution and adaptation of Fagaceae

The Fagaceae, a plant family with a wide distribution and diverse adaptability, has garnered significant interest as a subject of study in plant speciation and adaptation. Meanwhile, certain Fagaceae species are regarded as highly valuable wood resources due to the exceptional quality of their wood. In this study, we present two high-quality, chromosome-scale genome sequences for Quercus sichourensis (848.75 Mb) and Quercus rex (883.46 Mb). Comparative genomics analysis reveals that the difference in the number of plant disease resistance genes and the nonsynonymous and synonymous substitution ratio (Ka/Ks) of protein-coding genes among Fagaceae species are related to different environmental adaptations. Interestingly, most genes related to starch synthesis in the investigated Quercoideae species are located on a single chromosome, as compared to the outgroup species, Fagus sylvatica. Furthermore, resequencing and population analysis on Q. sichourensis and Q. rex. reveal that Q. sichourensis has lower genetic diversity and higher deleterious mutations compared to Q. rex. The high-quality, chromosome-level genomes and the population genomic analysis of the critically endangered Q. sichourensis and Q. rex will provide an invaluable resource as well as insights for future study in these two species, even the genus Quercus to facilitate their conservation.

Combined 2D- and 3D ERT monitoring as a geophysical tool for investigating spatial and temporal soil moisture fluctuations in a pine-beech forest

As climate change continues, forests are increasingly suffering from drought stress, which is leading to widespread forest dieback, but also to increased mortality of individual trees. In this regard, the impact of small-scale differences in water availability on individual trees has not yet been sufficiently studied to determine possible responses of different tree species to future droughts. Since conventional soil moisture monitoring and sampling methods only consider single points or small volumes, Electrical Resistivity Tomography (ERT) is becoming increasingly important to cover a larger survey area and to detect small-scale heterogeneities with regard to soil properties and soil moisture.The current study describes the application of a combined two- and three-dimensional geoelectrical monitoring approach with daily measurements over two years (May 2021 – April 2023) in a forest ecosystem in Lower Franconia (northwestern Bavaria, Germany), which is strongly affected by climate change. Soil water content, soil matric potential, throughfall, and stem flow are also measured at the forest site as well as precipitation at an adjacent forest clearing.The seasonally (long-term) and precipitation-driven (short-term) temporal change of soil resistivity is correlated strongly with the measured soil water content and matric potential. The applied 3D-ERT approach also allowed a first three-dimensional monitoring of the subsurface below a European beech located in the middle of the measuring grid with a daily resolution. The corresponding results also provide first indications that besides soil moisture changes also chemical processes in the subsurface may influence temporal resistivity changes in the soil of forest sites.The results of this study show that daily 3D-ERT is very suitable for investigating small-scale as well as short- and long-term variations in soil moisture, which is becoming increasingly important for understanding the causal relationships considering tree mortality and the subsurface.

Mode coupling and signal energy distribution in an open-grown European beech tree

The wind-induced response of the branches, the stem, and the roots of an open-grown European beech (Fagus sylvatica L.) was investigated to determine how the airflow’s kinetic energy transferred to the tree is dissipated. The instrumentation in the above-ground tree parts consisted of 69 Tree Response Sensors. The response of the primary roots to the wind load was recorded with six Tree Strain Sensors. The measurements were used to record the tree’s main vibration modes and quantify the energy dissipation from the measurement signals. The signal energy is reduced linearly by 90% from the crown periphery to the stem base. The base of the stem responds to higher wind loads than the branches and the upper part of the stem. The first-order mode frequency band is the narrowest in the roots. To dissipate the kinetic energy transferred to it as efficiently as possible, all tree parts vibrate in overlapping frequency bands in fundamental mode, resulting in mode coupling from the crown periphery to the roots. Mode coupling enables the elastic energy to be transferred as efficiently as possible via the roots to the ground to withstand wind loads and return to the resting position as quickly as possible.

Unpunctual in diversity: The effect of stand species richness on spring phenology of deciduous tree stands varies among species and years.

Climatic drivers alone do not adequately explain the regional variation in budburst timing in deciduous forests across Europe. Stand-level factors, such as tree species richness, might affect budburst timing by creating different microclimates under the same site macroclimate. We assessed different phases of the spring phenology (start, midpoint, end, and overall duration of the budburst period) of four important European tree species (Betula pendula, Fagus sylvatica, Quercus robur and Tilia cordata) in monocultures and four-species mixture stands of a common garden tree biodiversity experiment in Belgium (FORBIO) in 2021 and 2022. Microclimatic differences between the stands in terms of bud chilling, temperature forcing, and soil temperature were considerable, with four-species mixtures being generally colder than monocultures in spring, but not in winter. In the colder spring of 2021, at the stand level, the end of the budburst period was advanced, and its overall duration shortened, in the four-species mixtures. At species level, this response was significant for F. sylvatica. In the warmer spring of 2022, advances in spring phenology in four-species stands were observed again in F. sylvatica and, less markedly, in B. pendula but without a general response at the stand level. Q. robur showed specific patterns with delayed budburst start in 2021 in the four-species mixtures and very short budburst duration for all stands in 2022. Phenological differences between monocultures and four-species mixtures were linked to microclimatic differences in light availability rather than in temperature as even comparatively colder microclimates showed an advanced phenology. Compared to weather conditions, tree species richness had a lower impact on budburst timing, but this impact can be of importance for key species like F. sylvatica and colder springs. These results indicate that forest biodiversity can affect budburst phenology, with wider implications, especially for forest- and land surface models.

How beech provenance affects the structure of secondary xylem, leaf traits, and the ectomycorrhizal community under optimal growth conditions

Abstract Key message Provenance controls conductive area of stem secondary xylem, leaf area, and stable isotope (C, N, and O) ratios of beech leaves, while no significant effects were observed for ectomycorrhizal community composition. Abstract Beyond growth parameters and drought tolerance, comparatively little is known about the functioning of different beech (Fagus sylvatica L.) provenances. We investigated properties of leaves, stem secondary xylem, and ectomycorrhiza (ECM), and explored their interdependencies to identify the best performing beech provenance in optimal growth conditions. The study was conducted on 23-year-old trees in a provenance trial. The investigated provenances originated from Atlantic (Belgium—BE), Alpine (Italy—IT, Slovenia—SI), and continental climates (the Czech Republic—CZ). A significant effect of provenance was observed for stem vessel diameters and conductive area, as well as for foliar %C, δ13C, δ15N, and δ18O. δ13C as a proxy of intrinsic water use efficiency (iWUE) showed that the highest iWUE was achieved in BE provenance. Individuals with a better iWUE had wider growth rings regardless of provenance. Better iWUE was associated with lower specific leaf area (SLA). ECM community composition and diversity indices did not differ significantly among the provenances. Specific ECM taxa were associated with individuals with high SLA, δ13C, δ15N, and δ18O. In optimal growth conditions with no stress events, BE is a promising provenance due to an efficient water conducting system with high vessel diameters and conductive area, and high iWUE, while Alpine provenances showed an adaptation of their water conducting system to freezing conditions at their original locations. Integrating findings from different compartments improves our understanding of functioning of different beech provenances.

Influence of Fly Ash on Soil Properties and Vegetation of Fresh Coniferous Forest during Long-Term Observation

Fly ashes produced in huge amounts during coal combustion requires proper management. The purpose of this study was to determine the impact of fly ash from burning hard coal used in large doses (250, 500, 1000 and 2000 t ha−1) on soil properties and vegetation of fresh mixed coniferous forest within 43 years from the ash application. The experiment was established in the Podzols in the forest habitat of Czułów, Katowice Forest district, Upper Silesia, Poland. Eight tree species were planted in ridges created by ploughing: Pinus sylvestris, P. nigra, Larix decidua, Betula pendula, Quercus robur, Q., Acer pseudoplatanus and Fagus sylvatica. The changes in soil morphology caused significant transformations in the physical and chemical properties of the soil such as soil texture, pH, macronutrients (P, K and Mg) content and C:N ratio. Increasing of ash doses changed the granulometric composition of the soil levels from loamy sand (250 t/ha−1) to silt loam (2000 t ha−1). Initially, the acidic Podzols were alkalized under the influence of the fly ash and then acidified, possibly due to the impact of accumulated litter layers, and the reaction of organic soil horizons changed from strongly acidic (250–1000 t ha−1) to alkalis (2000 t/ha−1). The macronutrients content increased in proportion to the fly ash dose, but the subsequent acidification resulted in a gradual decrease in the macronutrients share in the soil layers. The value of the C:N ratio grew after the ash application and then it gradually reduced, even by half. The transformations of soil horizons’ properties also increased the capacity of the soil sorption complex (CEC). All these processes led to a change in the trophic status of the habitat expressed by the soil habitat index (SIG) and the initial coniferous forest site can be classified as a mixed forest habitat even with the lowest ash dose used. The composition of plant communities developed forty years after the ash application was similar at the lower ash doses and the most frequent and abundant tree species were L. decidua, P. nigra and P. silvestris. B. pendula was previously co-dominant, but it was eliminated from the tree stands during the experiment. Planted trees characteristic of late stages of succession, such as Q. robur, Q. rubra, F. sylvatica and A. pseudoplatanus either did not survive or remained in very low quantities. The herb and moss layers developed in the process of spontaneous colonization, and together with the trees led to phytostabilisation of the bare substrates. After acidification of the topsoil horizons, the herb layers consisted mostly of coniferous, mixed, and deciduous forest species, and the most frequent or abundant were Lysimachia europea and Pteridium aquilinum. The moss layers were represented by coniferous forest flora. At the ash dose of 2000 t ha−1, Tilia cordata settled in one of the seral stages of spontaneous succession and this species dominated in the community and formed a dense tree stand. After the soil acidification, a shift from calcicole to calcifuge plant strategy took place among species of the herbaceous layer. The transformations of plant communities’ composition occurred in relation to changes in the soil properties.

ASSESSING 14C BLANKS IN THE SMALL-SCALE ANALYSIS OF N-ALKANE COMPOUND-SPECIFIC-RADIOCARBON-ANALYSIS

ABSTRACT Compound-specific radiocarbon analysis (CSRA) provides the possibility to date sample material at a molecular level. N-alkanes are considered as specific compounds with high potential to CSRA. As these compounds originate from plant waxes, their radiocarbon (14C) analysis can provide valuable information about the age and origin of organic materials. This helps to reconstruct and understand environmental conditions and changes in vegetation in the past. However, CSRA has two main challenges: The small sample size of CSRA samples, making them extremely sensitive to blank effects, and the input of unknown amounts of extraneous carbon during the analytical procedure. According to the previous study from Sun and co-workers, we used different-sized aliquots of leaves Fagus sylvatica (nC27, nC29) and Festuca rubra agg (nC31, nC33) as modern standards and two commercial standards (nC26, nC28) as fossil standards for blank determination. A third commercial standard (nC27) with predetermined radiocarbon content of F14C = 0.71 (14C age of 2700 BP) serves to evaluate the blank correction. We found that the blank assessment of Sun and co-workers is also applicable to n-alkanes, with a minimum sample size of 15 µg C for dependable CSRA dates. We determined that the blank introduced during the analytical procedure has a mass of (4.1 ± 0.7) µg carrying a radiocarbon content of F14C = 0.25 ± 0.05. Applying the blank correction to a sediment sample from Lake Holzmaar (Germany) shows that all four isolated n-alkanes have similar 14C ages. However, the bulk material of the sediment and branches found in the sediment core are younger than the CSRA dates. We conclude that the disparity between the actual age of analysed organic material and the age inferred from radiocarbon results, which can occur in sediment traps due to delayed deposition, is the reason for the CSRA age.

The Effect of Rays on the Mechanical Behaviour of Beech and Birch at Different Moisture and Temperature Conditions Perpendicular to the Grain

The plastic deformation of wood perpendicular to the grain is gaining increasing importance due to advancements in forming technologies and the densification of wood. This study investigates how two hardwood species, i.e., beech (Fagus sylvatica) and birch (Betula pendula), respond to compression in the radial direction and examines the structural changes they undergo during both elastic and plastic deformation. Stress–strain curves at different moisture contents (dry to wet) and temperature conditions (20 to 140 °C) were recorded. In-situ observations at high moisture content and temperatures by means of different microscopic techniques are practically unfeasible. Therefore, the specimens were analysed ex-situ microscopically after the test. In addition to the compression of transversely oriented fibres and vessels, special attention was paid to the deformation behaviour of the wood rays. The results suggest that the wood ray cells carry a relatively higher proportion of the load in the radial loading direction than the surrounding vessels and fibres. This observation is supported by the higher percentage of deformed vessels, seen in the microscopy, in areas where the rays developed kinks, usually in the early wood of beech and anywhere in the cross-section of birch. The weaving of rays around big vessels introduced shear strains under compressive stresses at the kinked rays’ area. Thus, shear deformation is more evident in early wood than in late wood regions of wood. However, when the wood was tested at elevated moistures and temperatures, the material demonstrated a ductile response, namely the absence of localised shear deformations. Notably, wet beech and birch specimens heated to 100 °C and above exhibited pronounced thickness recovery and there was slightly irreversible buckling of rays and vessel deformations. Therefore, under such conditions, wood behaves like a “sponge” and is expected to be successfully processed without introducing clear damage to the material. This characteristic holds promise for replication in the development of bio-based energy-absorbing materials.

Xylem safety in relation to the stringency of plant water potential regulation of European beech, Norway spruce, and Douglas-fir trees during severe drought

Abstract Key message Norway spruce operates with larger hydraulic safety margins (HSM) than beech and Douglas-fir despite the known drought sensitivity of spruce, questioning a pivotal role of HSM in drought tolerance. Abstract The exceptional 2018/2019 drought exposed Central Europe’s forests to severe stress, highlighting the need to better understand stomatal regulation strategies and their relationship to xylem safety under extreme drought. We studied diurnal, seasonal, and inter-annual variation in stomatal conductance (gs) and leaf water potential (ΨLeaf) in co-occurring European beech (F. sylvatica), Norway spruce (P. abies), and Douglas-fir (P. menziesii) trees in the two summers and related them to hydraulic traits characterizing drought resistance. In 2018, F. sylvatica exhibited a continuous ΨLeaf decline from June to September, as is characteristic for an anisohydric strategy, while P. abies closed stomata early and reached the least negative ΨLeaf-values at the end of summer. P. menziesii showed low ΨLeaf-values close to P12 (the xylem pressure at onset of embolism) already in July. Both conifers closed stomata when approaching P12 and maintained low gs-levels throughout summer, indicative for isohydric regulation. In 2019, all three species showed a linear decline in ΨLeaf, but F. sylvatica crossed P12 in contrast to the conifers. The three species exhibited similar water potentials at turgor loss point (− 2.44 to − 2.51 MPa) and branch P50 (xylem pressure at 50% loss of hydraulic conductance; − 3.3 to − 3.8 MPa). Yet, F. sylvatica and P. menziesii operated with smaller hydraulic safety margins (HSM means: 0.79 and 0.77 MPa) than P. abies (1.28 MPa). F. sylvatica reduced leaf size and specific leaf area in 2019 and increased Huber value. Our species comparison during extreme drought contradicts the general assumption that conifers operate with larger HSMs than angiosperm trees. Contrary to expectation, P. abies appeared as hydraulically less vulnerable than Douglas-fir.

Seed Quantity and Quality Variation in European Beech (Fagus sylvatica L.)

European beech (Fagus sylvatica L.) is characterized by inter-annual cycles of seed production (masting), with synchronized high seed production between sites in some years. The importance of both seed quantity and quality monitoring stems from its role in sustaining the ecological balance, promoting biodiversity, and ensuring the long-term viability of forest ecosystems. This study aimed to (1) describe seed production from 2015 to 2022, (2) identify the potential factors that could affect the yield appearance and seed quantity, and (3) compare the quality of beechnuts from different crop years in Croatia. At each of the six forest seed objects located in the area of forest administrations Bjelovar, Karlovac, Ogulin, Požega, and Delnice, we selected 5 trees for seed collection. Seeds were collected with large PVC nets covering total projection area of the tree crowns. Seed quality testing was performed according to the International Rules for Seed Testing (ISTA Rules). Our results indicate a consistent biennial beechnut cycle. The crop was completely absent in 2015, 2017, 2019 and 2020, while 2016, 2018, 2020 and 2022 were fruitful years. Weather plays a key role in triggering masting. We found that beechnut production is influenced by summer weather conditions (from June to August) in the previous two years. Abundance of beechnut production varies on an annual level, population level and on individual trees. Factors that partially explain the variability in yield are tree height and crown size. No significant differences in viability were found across different years and sites, though the results should be approached with caution due to limited sample representation. The study identified a positive correlation between seed weight and viability, as well as the impact of precipitation on moisture content. The results emphasize the need for continued monitoring and further research to understand the factors influencing beechnut production and quality, crucial for sustainable forest management in the face of climate change.